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human embryonic kidney cells hek 293 t  (ATCC)


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    ATCC human embryonic kidney cells hek 293 t
    Human Embryonic Kidney Cells Hek 293 T, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12175 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+hek+293t+17+cells/293T%2F17/pmc13220488-47-0-6
    Average 99 stars, based on 12175 article reviews
    human embryonic kidney cells hek 293 t - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Construct:

    Article Title: A metabolic shift to the serine pathway induced by lipids fosters epigenetic reprogramming in nontransformed breast cells
    Article Snippet: NLS-roGFP2-Orp1 and mito-roGFP2-Orp1 (puromycin as selection mark) plasmids were obtained from cloning the c-myc sequence or a mitochondrial targeting sequence (MTS) sequence, respectively, into the pEIGW roGFP2-ORP1 vector (Addgene, #64993). .. For lentivirus production, human embryonic kidney (HEK) 293T/17 cells (ATCC, #CRL-11268) in phenol red–free Opti-MEM (Gibco) were cotransfected with a construct of interest and packaging plasmids Gag-Pol 8.91 (Addgene, #187441) and VSV-G (Addgene, #8454) using Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Article Title: A metabolic shift to the serine pathway induced by lipids fosters epigenetic reprogramming in nontransformed breast cells.
    Article Snippet: The generated images were analyzed using FIJI ImageJ (1.53c). .. For lentivirus production, human embryonic kidney (HEK) 293T/17 cells (ATCC, #CRL- 11268) in phenol red–free Opti- MEM (Gibco) were cotransfected with a construct of interest and packaging plasmids Gag- Pol 8.91 (Addgene, #187441) and VSV- G (Addgene, #8454) using Lipofectamine 3000 (Thermo Fisher Scientific). ..

    Cell Culture:

    Article Title: Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease.
    Article Snippet: .. Human embryonic kidney (HEK) 293T/17 cells (CRL- 11268, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium ( #10313- 021, Gibco, Waltham, MA, USA) with 10% Serum Plus II (Sigma- Aldrich, St. Louis, MO, USA) supplemented with penicillin (100 U/ml), streptomycin (100 μg/ ml; #400- 109, Gemini Bioproducts, West Sacramento, CA, USA), and 2 μM l- glutamine (#25030- 081, Gibco), in a humidified incubator at 37°C with 5% carbon dioxide. ..

    Article Title: Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease
    Article Snippet: .. Human embryonic kidney (HEK) 293T/17 cells (CRL-11268, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium ( #10313-021, Gibco, Waltham, MA, USA) with 10% Serum Plus II (Sigma-Aldrich, St. Louis, MO, USA) supplemented with penicillin (100 U/ml), streptomycin (100 μg/ml; #400-109, Gemini Bioproducts, West Sacramento, CA, USA), and 2 μM l -glutamine (#25030-081, Gibco), in a humidified incubator at 37°C with 5% carbon dioxide. ..

    Modification:

    Article Title: Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease.
    Article Snippet: .. Human embryonic kidney (HEK) 293T/17 cells (CRL- 11268, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium ( #10313- 021, Gibco, Waltham, MA, USA) with 10% Serum Plus II (Sigma- Aldrich, St. Louis, MO, USA) supplemented with penicillin (100 U/ml), streptomycin (100 μg/ ml; #400- 109, Gemini Bioproducts, West Sacramento, CA, USA), and 2 μM l- glutamine (#25030- 081, Gibco), in a humidified incubator at 37°C with 5% carbon dioxide. ..

    Article Title: Strategy to overcome a nirmatrelvir resistance mechanism in the SARS-CoV-2 nsp5 protease
    Article Snippet: .. Human embryonic kidney (HEK) 293T/17 cells (CRL-11268, American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium ( #10313-021, Gibco, Waltham, MA, USA) with 10% Serum Plus II (Sigma-Aldrich, St. Louis, MO, USA) supplemented with penicillin (100 U/ml), streptomycin (100 μg/ml; #400-109, Gemini Bioproducts, West Sacramento, CA, USA), and 2 μM l -glutamine (#25030-081, Gibco), in a humidified incubator at 37°C with 5% carbon dioxide. ..

    Suspension:

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL-11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C-terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis.
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL- 11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C- terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Transduction:

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL-11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C-terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis.
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL- 11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C- terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Infection:

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL-11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C-terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis.
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL- 11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C- terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Incubation:

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL-11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C-terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..

    Article Title: Cryo-EM of autoantibody-bound NMDA receptors reveals antigenic hotspots in an active immunization model of anti-NMDAR encephalitis.
    Article Snippet: .. Briefly, human embryonic kidney (HEK) 293T/17 cells (American Type Culture Collection CRL- 11268) were grown in suspension and transduced with P2 BacMam viruses encoding the C- terminal truncated GluN1 (residues 1 to 847) and GluN2A (residues 1 to 866) at a multiplicity of infection of 2:2:1 (GluN1:GluN2A:cells) and incubated at 37°C. ..



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